A High Throughput Biochemical Fluorometric Method for Measuring Lipid Peroxidation in HDL

A High Throughput Biochemical Fluorometric Method for Measuring Lipid Peroxidation in HDL

Current cell-based assays for determining the functional properties of high-density lipoproteins (HDL) have limitations. We report here the development of a new, robust fluorometric cell-free biochemical assay that measures HDL lipid peroxidation (HDLox) based on the oxidation of the fluorochrome Amplex Red. HDLox correlated with previously validated cell-based (r = 0.47, p<0.001) and cell-free...

Title: A Fluorometric High-Throughput Assay for Measuring Chlamydial Neutralizing

23 Chlamydia trachomatis is an obligate intracellular mucosotropic pathogen that causes human 24 infections of global importance. C. trachomatis causes trachoma, the leading cause of 25 preventable blindness worldwide and is the most common cause of bacterial sexually transmitted 26 disease. Although oculogenital infections are treatable with antibiotics, a vaccine is needed to 27 control C. tr...

1 HDL , Lipid Peroxidation , and Atherosclerosis

Fluorometric method for measuring serum lipase activity.

We describe a method for determining lipase (triacylglycerol acyl-hydrolase, EC 3.1.1.3) activity in serum. Emulsified olive oil in tris(hydroxymethyl)aminomethane buffer is used as substrate. The course of the enzymatic reaction is followed by measuring the decrease in fluorescence with time of a fluorescent indicator, 4-methylumbelliferone, added to the substrate, caused by the change in the ...

A High Throughput Method for Measuring Polycyclic Aromatic Hydrocarbons in Seafood Using QuEChERS Extraction and SBSE

National Oceanic and Atmospheric Administration (NOAA) Method NMFS-NWFSC-59 2004 is currently used to quantitatively analyze seafood for polycyclic aromatic hydrocarbon (PAH) contamination, especially following events such as the Deepwater Horizon oil rig explosion that released millions of barrels of crude oil into the Gulf of Mexico. This method has limited throughput capacity; hence, alterna...